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Example of segmentation with Stardist model (A) Original image used for segmentation showing <t>DAPI-labeling</t> of nuclei. (B) Overlay of DAPI-staining and result of segmentation in red (step 22-c). The “Cell display” parameter selected here is “nuclei only” (step 22-d). Scale bar represents 200 μm.
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Example of segmentation with Stardist model (A) Original image used for segmentation showing <t>DAPI-labeling</t> of nuclei. (B) Overlay of DAPI-staining and result of segmentation in red (step 22-c). The “Cell display” parameter selected here is “nuclei only” (step 22-d). Scale bar represents 200 μm.
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Example of segmentation with Stardist model (A) Original image used for segmentation showing <t>DAPI-labeling</t> of nuclei. (B) Overlay of DAPI-staining and result of segmentation in red (step 22-c). The “Cell display” parameter selected here is “nuclei only” (step 22-d). Scale bar represents 200 μm.
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Example of segmentation with Stardist model (A) Original image used for segmentation showing <t>DAPI-labeling</t> of nuclei. (B) Overlay of DAPI-staining and result of segmentation in red (step 22-c). The “Cell display” parameter selected here is “nuclei only” (step 22-d). Scale bar represents 200 μm.
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Example of segmentation with Stardist model (A) Original image used for segmentation showing DAPI-labeling of nuclei. (B) Overlay of DAPI-staining and result of segmentation in red (step 22-c). The “Cell display” parameter selected here is “nuclei only” (step 22-d). Scale bar represents 200 μm.

Journal: STAR Protocols

Article Title: Protocol for studying the immune microenvironment of human hepatocellular carcinoma by Cell DIVE multiplex immunofluorescence imaging

doi: 10.1016/j.xpro.2025.103946

Figure Lengend Snippet: Example of segmentation with Stardist model (A) Original image used for segmentation showing DAPI-labeling of nuclei. (B) Overlay of DAPI-staining and result of segmentation in red (step 22-c). The “Cell display” parameter selected here is “nuclei only” (step 22-d). Scale bar represents 200 μm.

Article Snippet: Mounting medium: DAPI Fluoromount-G , SouthernBiotech , Cat#010-20.

Techniques: Labeling, Staining

Example of application of the classification model step 25 (A) mIF image showing CD3 (pink), CD31 (red) and CD68 (green) and DAPI (gray) staining. (B) Objects created by the segmentation and assigned to Lymphocytes CD3+ (pink), Cell endoth. CD31+ (red) or Macrophages CD68+ (green) classes by the classification model on image A. Scale bar represents 200 μm.

Journal: STAR Protocols

Article Title: Protocol for studying the immune microenvironment of human hepatocellular carcinoma by Cell DIVE multiplex immunofluorescence imaging

doi: 10.1016/j.xpro.2025.103946

Figure Lengend Snippet: Example of application of the classification model step 25 (A) mIF image showing CD3 (pink), CD31 (red) and CD68 (green) and DAPI (gray) staining. (B) Objects created by the segmentation and assigned to Lymphocytes CD3+ (pink), Cell endoth. CD31+ (red) or Macrophages CD68+ (green) classes by the classification model on image A. Scale bar represents 200 μm.

Article Snippet: Mounting medium: DAPI Fluoromount-G , SouthernBiotech , Cat#010-20.

Techniques: Staining

Example of final mIF image of human hepatocellular carcinoma whole slide For visualization purposes, only 5 markers are displayed: WGA (yellow), CD4 (orange), CD20 (green), CD31 (red), CD68 (blue) and DAPI (gray). Scale bar represents 2 mm.

Journal: STAR Protocols

Article Title: Protocol for studying the immune microenvironment of human hepatocellular carcinoma by Cell DIVE multiplex immunofluorescence imaging

doi: 10.1016/j.xpro.2025.103946

Figure Lengend Snippet: Example of final mIF image of human hepatocellular carcinoma whole slide For visualization purposes, only 5 markers are displayed: WGA (yellow), CD4 (orange), CD20 (green), CD31 (red), CD68 (blue) and DAPI (gray). Scale bar represents 2 mm.

Article Snippet: Mounting medium: DAPI Fluoromount-G , SouthernBiotech , Cat#010-20.

Techniques: